三倍体毛白杨无性系的AFLP分子标记鉴定

Molecular identification of Populus tomentosa triploid clones by AFLP

利用AFLP标记技术采用MseⅠ+3/EcoRⅠ+2引物组合,对12个三倍体毛白杨和二倍体毛白杨无性系进行了分析鉴定.从38对引物组合中选出12对扩增条带较多的引物组合,多态性比例最高的是M--CAT/E--TC,多态性水平达到32.4%,最低的M--CTT/E--TT只有15.7%,证明供试材料之间的亲缘关系较近;UPGMA聚类分析结果与供试材料的系谱关系以及形态鉴别结果一致,表明凡亲本相同或形态差异小的无性系具有较高的相似系数;利用3个引物组合的8条多态性条带构建了12个供试材料的指纹图谱,可以作为三倍体毛白杨无性系鉴别以及品种保护的依据.

Twelve Populus tomentosa triploids and diploids were identified by AFLP molecular technology using a Mse Ⅰ ＋ 3/EcoR Ⅰ ＋ 2 primer combination. Twelve primers with high amplified bands were selected from 38 primer combinations, including primer M-CAT/E-TC with the highest ratio of polymorphic sites （32.4%） as well as primer M-CTT/E-TT with the lowest ratio of polymorphic sites （15.7 % ）. The genetic relationships among the 12 materials are relatively close. The dendrogram result of 12 clones constructed with a UPGMA method is consistent with the pedigree relationship and morphological identification, which indicates that high similarity coefficients exist in the clones with the same parents or with small morphological differences. A fingerprinting map of 12 clones has been constructed by 8 polymorphic bands selected from 3 primer combinations, which can identify the true Populus tomentosa triploids and serve as the basis for species protection.