肾上腺髓质素在乳腺癌组织的表达及与临床病理特征的关系

The Expression of Adrenomedullin in Breast Cancer and Its Relationship with Clinicopathological Factors

目的:探讨肾上腺髓质素(adrenomedullin,ADM)在乳腺癌组织中的表达情况及其与临床病理指标的关系。方法:采用流式细胞术对32例乳腺癌组织、癌旁2cm及癌旁5cm乳腺组织和10例乳腺纤维腺瘤组织的ADM蛋白表达进行定量检测。结果:乳腺癌组织的ADM表达水平(1.234±0.456)高于癌旁2cm乳腺组织(1.011±0.336,P<0.05)和癌旁5cm乳腺组织(0.981±0.397,P<0.05),而癌旁2cm和癌旁5cm组织之间比较差异无统计学意义(P>0.05);乳腺癌组织的ADM表达水平也明显高于乳腺纤维腺瘤(0.930±0.141,P<0.01)。ADM在乳腺癌组织的表达水平与肿瘤的组织学分级相关(rs=0.552,P<0.01),组织学Ⅲ级的ADM表达水平(1.516±0.579)明显高于Ⅱ级(1.072±0.186,P<0.01)和Ⅰ级(0.956±0.223,P<0.05),Ⅱ级和Ⅰ级比较差异无统计学意义(P>0.05);乳腺癌组织的ADM表达水平与肿瘤大小、TNM分期、病理类型、淋巴结转移状况、ER/PR和C-erbB-2等临床病理指标无关联(P>0.05)。结论:ADM在乳腺癌组织的表达明显增高,肿瘤的组织学分化差者表达增强。

Objective： To investigate the expression of adrenomedullin （ADM） in human breast cancer and evaluate the relationship with the clinical figure. Methods： The quatity of ADM gene protein expression was detected by flow cytometry in thirty-two cases of breast cancer, whose tissues were two centimeter and five centimeter away from distal tumor margin and ten fibroadenoma tissues. Results： The expressional level of ADM in breast cancer tissues was significontly higher（ 1.234 ± 0.456） than those in two centimeter away （ 1.011± 0.336, P 〈 0.05）and five centimeter away （0.981 ± 0.397,P 〈 0.05）, and their difference had no statistical significance （P 〉 0.05）, the expression level of breast cancer was significenty higher than those in the flbroadenoma （0.930 ± 0.141,P 〈 0.01）. The expression level of ADM in the breast cancer was obviously related with the histological stage of tumor（r,= 0.552,P 〈 0.01）. The histological expression level of ADM in grading Ⅲ （1.516±0.579） was higher than those in grading Ⅱ （1.072 ± 0.186,P 〈0.01）and grading Ⅰ （0.956 ± 0.223,P 〈 0.05）.There was no significance statistical between grading Ⅱ and grading Ⅰ（P 〉 0.05）. There wre no relationships between the expression level of ADM in cancer and tumor size, TNM stage, histological type, nodal status, EP/PR and c-erbB-2 （P 〉 0.05）. Conclusion： The expression of ADM signifcantly increase in breast cancer and the worse histologial differentiation expression increase.