NO和iNOS在肝硬化患者肝组织中的表达及与门静脉压力的关系

Study on the Relationship between Potal Pressure and the Expression of iNOS and NO in Liver of Patients with Hepatic Cirrhosis

目的:观察NOS-NO系统在肝硬化患者肝组织中的表达及与门静脉压力的关系,以探讨其在肝硬化门脉高压中的作用。方法:随机抽取20例正常志愿者及20例肝硬化患者,在B超引导下经皮经肝穿刺分别测定门静脉压力、抽取门静脉血和外周血并留取肝组织,测定血液中NO浓度,观察肝组织iNOS的表达。结果:肝硬化患者下腔静脉及门静脉血中NO浓度、肝组织iNOS的表达及门静脉压力均分别显著高于正常对照组。正常对照组的外周血和门静脉血中NO浓度水平接近,差异无统计学意义;但肝硬化患者的门静脉血NO浓度显著高于外周血NO浓度。门静脉压力与外周静脉血NO浓度、门脉血NO浓度及肝组织中iNOS的表达呈直线相关关系。结论:门静脉压力与门脉血NO浓度、肝组织中iNOS的表达密切相关。

Objective： To observe the relationship between the expression of NOS-NO system in the liver of patients with hepatic cirrhosis and portal pressure and to investigate its action on the portal hypertension. Methods： Twenty normal volunteers and twenty patients with hepatic cirrhosis were chosen randomly and liver puncture was given through skin under the direction of B-ultrasonic equipment to examine portal pressure, portal vein and peripheral blood were drawed and the liver tissue was taken respectively in order to measure the density of blood NO and study the expression of the liver iNOS. Results： The density of NO in inferior and portal vein blood, expression of the liver iNOS and the portal pressure in the patients with hepatic cirrhosis were all significantly higher than that of the control group. The level of NO had no obvious difference between peripheral and portal vein blood in the control group, but NO density of portal vein in the hepatic cirrhosis was significantly higher than that of the peripheral blood. The portal pressure and NO density in peripheral vein blood,NO level in the portal blood and iNOS expression in the liver tissue had linear correlation. Conclusion： The portal pressure is closely related to NO density in portal vein, INOS expression and in the liver tissue.