淫羊藿甙对OVX大鼠成骨细胞Smad4 mRNA的作用

An Experimental Study of Effects of Icariin on Increasing Smad4 mRNA Level in Osteoblast Cells of OVX Rats

目的:探讨淫羊藿甙(icariin)抑制骨质疏松的细胞内信号机制。方法:36只雌性Wistar大鼠随机分成3组:OVX(双侧卵巢切除);OVX-I(OVX并注射icariin);OVX-E(OVX并注射雌二醇)。术后4,6,8周时检测血清碱性磷酸酶(ALP)的含量,并于术后8周时测体质量,取股骨干分离培养成骨细胞,用RT-PCR检测各组成骨细胞Smad4的mRNA含量。结果:(1)Icariin可以控制OVX大鼠体质量增加。(2)术后4,6周OVX-I和OVX-E组血清ALP值升高差异无统计学意义;OVX组ALP升高值与其他2组间差异有统计学意义(P<0.01);术后8周OVX、OVX-I和OVX-E组血清ALP值依次降低,差异有统计学意义(P<0.01)。(3)术后8周,RT-PCR显示OVX、OVX-I和OVX-E组Smad4的mRNA含量依次增高,以OVX-E组最多。结论:淫羊霍甙可抑制OVX大鼠血清ALP水平的升高,且可能通过提高Smad4mRNA抑制OVX大鼠体质量的增加。

Objective： To explore the cell signal mechanism of osteoporosis prevented by icariin. Methods： thirty-six female wirst rats were randomly divided into 3 groups： the OVX group（ovariectomize）; the OVX-I group（OVE and treated with icarrin） and the OVX-E group （OVE and treated with estradiol）. The plasma alkaline phosphatase （ALP） was determined four, six, eight weeks after the surgery and the animal body weight was measured eight weeks after the surgery then the shaft of femur was separated and cultured tothe osteoblast. RT-PCR was employed to detect the Smad4 mRNA level of osteoblast in all three groups. Results： （1）The body weight increase could be inhibited by injection of icariin.（2）The increase of ALP level in the OVX- I and OVX-E group had no statistical significance four, six weeks after surgery but ALP increase in the OVX group was statistically significant compared with two others （P 〈 0.01）, the ALP level decreased in onoler of the OVX, OVX-I and OVX-E group eight weeks after surgery, the difference was statistically significant （P 〈 0.01）.（3）The level of the Smad4 mRNA increased in order of the OVX, OVX-I, OVX-E group detected by RT-PCR eight weeks after surgery, especially in the OVX-E group. Conclusion： Icarrin can inhibit the amount of ALP increase in the OVX rat and reduce the rat body weight by increasing Smad4 mRNA.