摘要
以p3301-BI121质粒为基础,通过中间载体pGEX-KG,构建了由CaMV35S启动子调控的PpDREB2基因植物表达载体p3301-BI121-PpDREB2,选择标记为PPT(Phosphinothricin),通过冻融法将重组质粒导入根癌农杆菌EHA105, 为通过根癌农杆菌介导法将PpDREB2基因导入植物奠定基础。
The plant transformation vector p3301-BI121-PpDREB2 was constructed in this experiment. The PpDREB2 gene was cloned into pGEX-KG vector firstly, and then was ligated to p3301-BI121 vector. The gene was controlled by CaMV35S promoter and the selective marker gene was PPT （phosphinothricin）. Then the recombinant plasmid was transferred into Agrobacterium Tumefaciens EHA105 by direct entering way.
出处
《华北农学报》
CSCD
北大核心
2006年第2期18-22,共5页
Acta Agriculturae Boreali-Sinica
基金
国家863计划项目(2002AA22409)
