甘蓝型黄籽油菜分子标记辅助选择的效果分析

The Effects Analysis of Molecular Marter-assisted Selection in Yellow-seeded Brassica napus

应用分子标记辅助选择的方法,将黄籽性状导入到波里马恢复系中,利用杂种优势,将是提高油菜产量的理想途径。分析了分子标记辅助选择甘蓝型黄籽油菜的前景和背景选择的效果,结果表明,在背景选择中,7种不同 RAPD引物数目组合方式的选择结果基本一致;应用少数连锁群上的标记可以获得与全基因组相似的选择结果。在前景选择中,随着世代的增加,每一个标记的选择准确率在降低;双侧标记的选择准确率比单侧的标记高,此外,多个单侧标记未能提高选择准确率。据此,提出了作物前景和背景选择过程中的一些改进策略。

To transfer the yellow-seeded gene into Pol cms restorer lines and utilize heterosis using molecular marterassisted selection （MAS） would be an ideal approach to increase the rapeseed oil yield. This paper analyzed the effects of the back and foreground selection in yellow-seeded Brassica napus, the results showed that the selection results of 7 different RAPD primer combinations are identical and selection by a limited linkage groups can get the whole genome similar effect in background selection. During foreground selection program, it demonstrated that the selection efficiency of each marker would decrease with generation and the unilateral markers can＇t increase the efficiency with number, moreover, flanked markers can attain higher efficiency than unilateral markers. As a result, we proposed some improved strategies for back and foreground selection in crop MAS.