摘要
利用C18色谱柱,以甲醇、水、冰乙酸(45:54．8:0．2)为流动相干255 nm检测细菌培养液提取物,根据HPLC检测结果快速筛选得到既产赤霉素(GA3)又产生长素(IAA)的细菌1株CX-5-2,并利用:HPLC进一步研究并明确了该菌株不产植物激素的适宜条件。同时明确了该菌株对小麦生长具有促进的潜力。
The anxin including GA3 and IAA in the culture broth of 300 bacterial isolates were measured through HPLC in this study, and an isolate CX-5-2 were screend and determined to prorduce GA3 and IAA. Simultaneously, the optimum conditions of strain CX-5-2 to produce auxin were studied through HPLC. The results from the experiment on effects of strain CX-5-2 culture broth on the groth of wheat seedlings determined that this bacterial strain has the potential ability of plant growth-promotion.
出处
《华北农学报》
CSCD
北大核心
2006年第2期66-69,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省农林科学院重点项目资助(A03-1-03-09)
