中国沙棘和俄罗斯沙棘在不同土壤水分条件下保护酶系统和丙二醛的变化

Changes in Protective Enzymes and Malondiadehyde in Leaves of Chinese Sea Buckthorn and Russian Sea Buckthorn under Different Soil Water Condition

在野外不同生境和田间不同干旱条件下对中国沙棘(Hippophae rhamnoides sinensis)和俄罗斯沙棘(Hippophae rhamnoides)的SOD,CAT和MDA进行研究后发现,野外沟底土壤水分含量显著高于坡上土壤水分含量,因此,沟底中国沙棘叶片的SOD,CAT活性高于坡上,MDA含量则为坡上植株高于沟底植株。田间不同干旱胁迫条件下,随着土壤含水量的降低,沙棘叶片中的SOD活性在胁迫初期显著降低,随着干旱胁迫时间的延长持续降低,沙棘叶片SOD活性表现为中国沙棘雄株>俄罗斯沙棘雌株>俄罗斯沙棘雄株>中国沙棘雌株;CAT活性在胁迫初期有所升高,之后开始逐渐下降,种间的差异为中国沙棘高于俄罗斯沙棘,雌、雄株的差异为雌株高于雄株;膜脂过氧化物质MDA的含量随着干旱胁迫程度的增加和胁迫时间的延长持续增加,雌株叶片内MDA含量高于雄株,中国沙棘的MDA含量高于俄罗斯沙棘。

Through analyzing the changes of protective enzymes activity and malondiadehyde content in leaves of Chinese sea buckthorn and Russian sea buckthorn in field drought stress, the results showed that the slope soil＇s water content is significantly lower than the gully, so SOD activity and CAT activity in leaves of Chinese sea buckthom in the slope decreased than that in the gully, MDA content in leaves of Chinese sea buckthom in the slpoe increased than that in the gully. Under field drought stress, SOD activity in leaves of sea buckthorn decreased notablly in the initial stage of drought stress, then decreased with the prolonging of the drought stress time, SOD avtivity showed an order of male Chinese sea buckthom 〉 female Russian sea buckthom 〉 male Russian sea buckthom 〉 female Chinese sea buckthom. CAT activity increased in the initial stage of drought stress, then decreased after the initial stage, the difference between species was Chinese sea buckthom higher than Russian sea buckthom, the difference between male and female was the male higher than female . The content of MDA increased with decreasing of water content of the soil, MDA content in leaves of female higher than that of male, MDA content in leaves of Chinese sea buckthorn higher than that of Russian sea buckthorn.