摘要
胚胎干细胞具有的独特特征———分化全能性是生物学各研究领域多年来的热点之一。胚胎干细胞独特的生物学特征在生殖细胞与体细胞及动物个体之间建立了广泛的联系。胚胎干细胞应用前景有着巨大的潜力。为此,对其培养建系的完善性研究显得格外重要。在此,对胚胎干细胞建系方法方面做一简要综述,尤其对建系过程中内细胞团的分离方法及干细胞集落的获取方法进行探讨。
Embryonic Stem （ES） Cell which posses the omnipotence and the ability to proliferate indefinitely in vitro,The special feather is the Hotspot of biological research field. Embryonic Stem （ES） Cells connect somatic cell and germ cell with individual animal . that they have great applications perspective in physic. Culture and establish the ES cell lines is becoming important as the widely used of ES cells. This article gives a summary in the method of establishing the ES cell lines, especially in the isolation of the ICM and the ES clones,
出处
《生物技术通报》
CAS
CSCD
2006年第1期38-41,共4页
Biotechnology Bulletin
