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适合原位RT-PCR的石蜡切片制作方法 被引量:7

Research of the Paraffin Method Suits in Situ RT-PCR
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摘要 石蜡切片是原位RT-PCR中常用一种生物制片技术,简要阐述原位RT-PCR的原理以及适合于原位RT-PCR的石蜡切片的制作流程,对其易出现的问题和解决办法作了简要的概述。 The paraffin slice was a biological slice making technology commonly used in In situ RT-PCR, This article briefly elaborated the In situ RT-PCR principle, as well as the appropriate flow of paraffin slice making for the In situ RT-PCR. And the questions that were easy appeared in the paraffin making and the solutions were brief elaboration.
出处 《生物技术通报》 CAS CSCD 2006年第1期58-60,共3页 Biotechnology Bulletin
基金 国家自然科学基金资助项目(30060053 30360066) 国家科技攻关计划引导项目(2003BA546C) 兵团科委项目(NKB02SDXNK01SW)
关键词 石蜡切片 原位RT—PCR Paraffin slice In situ RT-PCR
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  • 1汪谦.现代医学实验方法[M].北京:人民卫生出版社,1998.914.
  • 2王伯云.病理学技术[M].北京:人民卫生出版社,2000.565-594.
  • 3Bagasra O, Sheshamma T, Haansen J, et al. Application of in situ PCR methods in melocular biology. Special applications in electron microscopy,cytogenetics and immmohistochemistry. Cell Vision, 1994,2: 621 - 70
  • 4Berger MM, See DM, Redl B , et al. Comparison of procedures for the detection of enteroviruses in murine heart samples by in situ polymerase chain reaction. Res - Virol, 1997,148:409 - 416
  • 5Cho NH, Joo HJ, Ahn HJ, et al. Detection of human papillomavirus in warty carcinoma of the uterine cervix: comparison of immunohisto - chemistry, in situ hybridization and in situ polymerase chain reaction methods.Pathol Res Pract, 1998,194:713 - 720
  • 6Komminoth P, Long AA. In - situ polymeraes chain reaction. An overview of methods applications and limitations of a new molecular technique. Virchows Arch, 1993, B64:67 - 73
  • 7Hofler H,Puta B,Mueller JD, et al.In situ amplification of measles virus RNA by the self - sustained sequence replication reaction. Lab - Invest,1995,73: 577 - 585
  • 8Hoyland JA, Mee AP, Baird P, et al. Demonstration of estrogen receptor Mrna in bone using in situ reverse- transcriptase polymerase chain reaction. Bone, 1997,20: 87 - 92
  • 9Nuovo GJ, Gorgone GA, MacConnell P , et al. In situ localization of PCR - amplified human and viral cDNAs. PCR Methods Appl. 1992,2:117-123
  • 10Chen RH, Fuggle SV. In situ cDNA polymerase chain reaction: research and clinical. In: Kirkam N eds. Progres in Pathology. Vol 2. London UK:Churchill Livingstone, 1995.203 - 218

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