纤维素酶在甲壳胺上的固定化

Study on Chitosan-immobilized Cellulase

本文报道从虾壳中制取甲壳胺，并以甲壳胺为载体，戊二醛为交联剂，将纤维素酶［ＥＣ．３·２·１·４］固定化的研究结果．探讨了一定量湿甲壳胺载体与交联剂浓度、给酶量的最适固定化条件．同时对固定化酶及游离酶的米氏常数、最适ｐＨ、离子强度对酶活力的影响以及最适反应温度和热稳定性等理化性质进行了探讨和比较．

This paper deals with the preparation of chitosan from prawn shells and the result of the cellulase was immobilized on chitosan obtained (as a support) by a glutaraldehyde (as a cross-linking reagent).The best conditions of immobilization,such as the weights of wet chitosan and cellulase,and the concentration of glutaraldehyde,were studied. Some characters of immobilized and native cellulases,such as Michaelis contant,optimum pH,and the effect of ionic strength, optimum temperature, stabilities to heat and repeat operation were studied and compared.