甘薯薯瘟病原细菌对甘薯植保素的诱导初探

Preliminary Study on Phytoalexin Induced from Pseudomonas solanacoarum in Ipomoea batatas

甘薯（Ｉｐｏｍｏｅａｂａｔａｔａｓ）抗病品种＂湘薯６号＂和感病品种＂新种花１８３＂经薯瘟病原细菌（ＰｓｅｕｄｏｍｏｎａｓｓｏｌａｎａｃｏａｒｕｍＥ．Ｆ．Ｓｍｉｔｈ）的诱导，９０％甲醇提取后，用乙酸乙酯萃取和ＳｅｐｈａｄｅｘＬＨ－２０柱层析分离纯化得到４个吸收峰．经抑菌试验检测出＂湘薯６号＂的抑菌活性区在第２峰，而＂新种花１８３＂的抑菌活性区在第３峰．硅胶Ｇ２５４薄板层析法鉴定该提取物可能是植保素．紫外光谱、红外光谱分析表明该植保素的吸收峰与黄酮类化合物的特征吸收峰相似，可能属于黄酮类化合物．

The phytoalexin from sweet was dealed with. Spraying the solution of Pseudomonas solanacoarum(E. F. Smith)on the leaves of both resistent (Xiang Su 6)and susceptible(Xing Zhong Hua 183)varieties,we collected the leaves of both respectively.Dealing with by 90% methanol and extracting by ethyl acetate,then separating by chromatography on Sephadex LH-20 column,we obtained four peaks respectively. The bacteriostatic experiment turn out that the active area of resistant variety (Xiang Su 6)was in the second peak, but the susceptible variety (Xing Zhong Hua 183) was in the third peak. It was identified by the thin layer chromatography on silica gel G254 that the extraction was a phytoalexin. The analysis of UV and IR spectrum showed that this phytoalexin belongs to the derivate of isonavones.