苦参碱对P物质诱导HaCaT细胞表达IFN-γ和IL-10的影响

Effect of matrine on the expression of IFN-γ and IL-10 induced by substance P in HaCaT cells

目的:研究苦参碱对P物质诱导表皮角质形成细胞系HaCaT细胞表达IFN-γ和IL-10的影响。方法:采用ELISA法测定P物质诱导HaCaT细胞分泌IFN-γ和IL-10的时效关系与量效关系。以P物质刺激HaCaT细胞,加入不同剂量的苦参碱共孵育24 h,测定苦参碱对IFN-γ和IL-10表达的影响。结果:以1×10-8mol/L P物质刺激HaCaT细胞24 h,可以显著增加HaCaT细胞IFN-γ和IL-10的分泌量,不同浓度的苦参碱均可以促进HaCaT细胞分泌IFN-γ,其中50μg/mL和100μg/mL的苦参碱均可以显著增加IFN-γ的分泌量;但苦参碱对P物质诱导的IL-10的分泌无显著影响。结论:苦参碱可能通过促进抗炎因子IFN-γ的分泌而发挥抗皮肤变态作用。

Objective： To investigate the effect of matrine on the expression of IFN-γ and IL-10 induced by substance P in kcratinocytes line HaCaT cells. Methods： The time course and dose-response relationship of releasing IFN-γ and IL-10 in HaCaT cells stimulated by substance P were determined by ELISA method. The effect of matrine on the release of IFN-γ and IL-10 in HaCaT cells was also measured by EI.ISA kit. Results： The expression of IFN-γ and IL-10 in HaCaT cells stimulated by 1×10^-8 mol/L substance P for 24 h was increased significantly. Matrine （50 μg/ml. and 100μg/mL） significantly induced the production of IFN-γ stimulated by substance P but had no significant effect on the production of IL-10. Conclusion： The in hibitory effect of matrine on the cutaneous allergy may be accomplished by inducing the production of IFN-γ.