HPLC法测定关节腔引流液中头孢拉定的浓度

Determination of the content of cefradine in drainage-fluid from articular cavity by HPLC method

目的：建立简便快速的HPLC法，测定髋关节腔引流液中头孢拉定的药物浓度。方法：采用Hypersil C18（250mm×4．6mm，5μm）色谱柱，流动相为甲醇-0．02mol／L的醋酸钱（25：75），流速1.0mL／min，检测波长为254nm，测定关节腔引流液中头孢拉定的药物浓度。结果：关节腔引流液标本中头孢拉定的线性范围分别为5～50μg／mL（r=0．9994）和51～500μg／mL（r=0．9999），回收率（99．08±2．08）％，日内RSD为1．82％，日问RSD为2．10％，回收率和精密度均符合要求。结论：该方法简便、准确，可用于临床标本的测定。

Objective： To establish a simple and quick method for determination of the concentration of cefradine in drainage-fluid from articular cavity by HPLC method, Methods： The drainage-fluid samples were eluted with methanol 0.02 mol/L ammonium acetate solution （25 ： 75） on a Hypersil C18 （250 mm）〈 4.6 mm, 5μm） column and the detection wavelength was 254 nm. The flow rate was 1.0 mL/min, Results： The linear ranges of cefradine in drainage-fluid were 5-50μg/mL （r=0.999 4） and 51-500μg/mL （r=0. 999 9）, The average recovery rate was （99.08±2.08）%. The intra-day and inter day RSDs were 1.82% and 2.10%, respectively, The average recovery rate and RSD fulfilled the requirements, Conclusion： The method is simple,accurate and suitable for the determination of the concentration of cefradine in drainage-fluid from articular cavity.