榼藤子水溶性提取物对小鼠移植瘤S_(180)的抑制作用

Antitumor Effects of Water Soluble Extracts from Entada on Transplant Tumor S_(180) of Mice

目的:研究榼藤子水溶性提取物对小鼠移植瘤S180的抑瘤作用及对血白细胞的影响。方法:昆明种小鼠腋部皮下接种S180瘤细胞后随机分组,分别灌胃给榼藤子水溶性提取物50,100,200 mg·kg-1和5-氟尿嘧啶(5-Fu)25 mg·kg-1以及0．9％氯化钠溶液,连续10 d,观察实验鼠的一般状况,瘤体性状、大小及组织学特征,计算抑瘤率,检测血白细胞。结果:榼藤子水溶性提取物对S180的抑瘤率在32．43％-47．75％之间,与0．9％氯化钠溶液组比较有显著性差异(P<0．05或P<0．01),且抑瘤率与给药剂量呈正相关。病理组织学观察表明:试验组瘤块中央片状坏死,周围有大量的淋巴细胞、巨噬细胞浸润。榼藤子水溶性提取物对小鼠的外周血白细胞没有明显影响(P>0．05)。结论:榼藤子水溶性提取物对S180有良好的抑制作用,对外周血白细胞没有明显影响。

Objective ： To study the inhibitory effects of water soluble extracts from Entada on transplant tumor S180 of mice and the influence with the number of blood white cell of the mice. Method： The model mice were treated with the water soluble extracts from Entada（50,100,200 mg·kg^-1 ） ; 5-Fu（25mg·kg^-1 ） ; and 0.9% sodium chloride by gastrogavage, for ten days. The pill,body weight,locomotion were observed, then tumor bodies were stripped and histop.athological detects were adopetod with HE staining . The rates of inhibition tumor were calculated, and the number of blood white cell was also calculated. Result： The rates of inhibition tumor of water soluble extracts from Entada on Sis0 were 32.43%-47.75%. To compare with solvent groups, the rates of inhibition tumor were higher obviously （P 〈 0.05 or P 〈 0.01 ）and had positive correlation with drug dose. Histopathological detects demonstrated that tumor body of experiment group had excessive patch necrosis in its central position and had a number of infiltration of lymphocytes and macrophages in its peripheral portion. Water soluble extracts from Entada had no obvious influence with the number of blood white ceil of the mice（P 〉 0.05）. Conclusion： Water soluble extracts from Entada had good inhibition effect on S18o and had no obvious influence with the number of blood white cell of the mice.