辛伐他汀对PTHrP诱导的破骨细胞性骨吸收及小鼠颅盖骨代谢的影响

Effect of simvastatin on the osteoclastic resorption stimulated by PTHrP and anabolism with murine calvarial organ culture in vitro

[目的]探讨辛伐他汀对体外甲状旁腺素相关肽(PTHrP)诱导小鼠的破骨细胞骨吸收功能的作用及其小鼠骨代谢的影响。[方法]采用PTHrP诱导小鼠骨髓细胞培养破骨细胞和小鼠颅盖骨培养体系,检测辛伐他汀作用8 d后破骨细胞骨吸收陷窝和培养上清钙的变化;检测小鼠颅盖骨培养上清碱性磷酸酶和钙含量,组织学观察小鼠颅盖骨形态学变化。[结果]辛伐他汀体外可明显抑制PTHrP诱导小鼠的破骨细胞骨吸收陷窝的形成及培养上清钙的释放,辛伐他汀体外可增强小鼠颅盖骨培养上清碱性磷酸酶的活性,组织学观察到辛伐他汀使小鼠颅盖骨矿化增强。[结论]辛伐他汀体外不仅可促进小鼠颅盖骨的成骨活性,并且可明显抑制PTHrP诱导小鼠的破骨细胞骨吸收功能,对骨吸收性疾病有着重要的防治作用。

[ Objective] To study the effect of simvastatin in the osteoclastic resorption stimulated by PTHrP and murine bone anabolism in vitro. [ Method ] The bone resorption activities of the osteoclast stimulated by PTHrP were evaluated after treatment with simvastatin for 8 days in vitro; the concentration of Ca^2＋ in the supematant was also detected by atomic absorption spectrometer. The concentration of ALP and Ca^2＋ of the supematant in murine calvarial organ culture were detected. The histology of ealvaria was observed. [ Result] Simvastatin greatly inhibited the osteoclastic bone resorption stimulated by PTHrP in vitro and reduced the release of Ca^2 ＋. Simvastatin increased the ALP activities and bone mineralization of murines calvarial organ culture in vitro. [Condusion] Simvastatin may inhibit the osteoclasric resorption stimulated by PTHrP and promote osteoblast differentiation and bone mineralization in vitro, thus play an important role in the prevention and treatment of osteoporosis.