期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Nogo-C对PC12细胞分化和突起生长的影响 被引量：2

Effects of Nogo-C on differentiation and neurite extension of PC12 cells
下载PDF
导出
摘要 目的:利用PC12细胞研究Nogo-C和神经元细胞分化及突起生长的关系.方法:NGF诱导PC12细胞,利用RT-PCR及免疫荧光技术检测细胞内Nogo-C的表达,并在PC12细胞中过表达Nogo-C,通过细胞计数检测Nogo-C对突起生长的影响.结果:①RT-PCR检测到NGF诱导的PC12细胞中有少量Nogo-C的mRNA分子表达;②免疫荧光染色观察到NGF诱导的PC12细胞中有少量Nogo-C的表达;③细胞计数观察到转染Nogo-C后的PC12细胞在NGF诱导后突起增长百分数相对于EGFP组明显增高.结论:NGF诱导的PC12细胞中有Nogo-C的表达,同时过表达Nogo-C可促进NGF诱导下的PC12细胞的分化以及影响细胞的突起生长. AIM： To study the effects of Nogo-C on the differentiation and neurite extension of PC12 cells. METHODS： The expression of Nogo-C in NGF-treated PC12 cells was detected using RT-PCR and immunofluorescence. And pEGFPN1-Nogo-C, the GFP-tagged Nogo-C vector was transfected into PC12 cells. The effects of Nogo-C on the differentiation and neurite extension of PC12 cells were further studied by cell counting. RESULTS： A small amount of Nogo-C mRNA could be detected in NGF-treated PCI2 cells; Nogo-C was found to be expressed in NGF-treated PC12 cells by immunofluorescence; the neurlte extension of NGF-treated PC12 cells transfected with Nogo-C was significantly increased according to the results of cell counting. CONCLUSION： Nogo-C was expressed in NGF-treated PC12 cells, and overexpressed Nogo-C would facilitate the differentiation and influence the neurite extension of NGF-treated PC12 cells.
作者 武肖娜 李容 李静雯 鞠躬
机构地区 第四军医大学全军神经科学研究所
出处 《第四军医大学学报》 北大核心 2006年第8期723-725,共3页 Journal of the Fourth Military Medical University
基金 国家重点课题973项目资助(2003CB515301)
关键词 过表达 Nogo-C PC12细胞 细胞分化 overexpression Nogo-C PC12 cells cell differentiation
分类号 R338 [医药卫生—人体生理学]
  • 相关文献

参考文献11

  • 1Schwab ME. Nogo and axon regeneration [J]. Curr Opin Neurobiol,2004,14:1 -7.
  • 2He Z, Koprivica V. The Nogo signaling pathway for regeneration block EJ]. Annual Rev Neuro Sci, 2004,27:341 -368.
  • 3Huber AB, Weinmann O, Brosamle C, et al. Patterns of Nogo mRNA and protein expression in the developing and adult rat and after CNS lesions[J]. J Neuro Sci, 2002,22 (9) :3553 - 3567.
  • 4Jin WL, Liu YY, Liu HL, et al. Intraneuronal localization of Nogo-A in the rat[J]. J Comp Neurol, 2003,458(1) :1 -10.
  • 5Fouruier AE, Gould GC, Liu BP, et al. Truncated soluble Nogo receptor binds Nogo-66 and blocks inhibition of axon growth by myelin[J]. J Neuru Sci, 2000,22(20) :8876 -8883.
  • 6Chen MS, Hnher AB, van der Haar ME, et al. Nogo-A is a myelinassociated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1 [ J ]. Nature, 2000,403 (6768) :434 - 439.
  • 7GrandPre T, Nakamura F, Vartanian T, et al. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein [ J]. Nature, 2000,403(6768) : 439 -444.
  • 8Prinjha R, Moore SE, Vinson M, et al. Inhibitor of neurite outgrowth in humans [J]. Nature, 2000,403(6768) : 383 -384.
  • 9Zheng B, Ho C, Li S, et al. Lack of enhanced spinal regeneration in Nogo-deficient mice [ J ]. Neuron, 2003,38 (2) :2.
  • 10Rudkin BB, Lazarovici P, Levi BZ, et al. Cell cycle-specific action of nerve growth factor in PC12 cells: Differentiationwithout proliferation [J]. EMBO J, 1989,8:3319 -3325.

同被引文献31

  • 1Schwab ME, Thoenen H. Dissociated neurons regenerate into sciatic but not optic nerve explants in culture irrespective of neurotrophic factor [ J], J Neurosci, 1985, 5 (9) :2415-2423.
  • 2Caroni P, Schwab ME. Two membrane protein fractions from rat central myelin with inhibitory properties for neurite growth and fibroblast spreading [ J ]. J Cell Biol, 1985, 106 (4) : 1281- 1288.
  • 3Chen MS, Huber AB, Van-der-Haar ME, et al. Nogo-A is a myelin associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1 [J].Nature, 2000, 403 (6768) : 434-439.
  • 4Li M, Song J. The N- and C-termini of the human Nogo molecules are intrinsically unstructured: bioinformatics, CD, NMR charac terization, and functional implications [ J]. Proteins, 2007, 68 (1) :100-108.
  • 5Schiff D, Brown PD, Giannini C. Outcome in adult low-grade gliomas: the impact of prognostic factors and treatment [ J]. Neurology, 2007, 69 ( 13 ) : 1366-1373.
  • 6Li Q, Qi B, Oka K, et al. Link of a new type of apoptosis-inducing gene ASY/Nogo-B to human cancer[J]. Oncogene, 2001, 20(30) : 3929-3936.
  • 7Xiong NX, Zhao HY, Zhang FC, et al. Negative correlation of Nogo-A with the malignancy of oligodendroglial tumor[J].Neurosci Bull, 2007, 23( 1 ) :41-45.
  • 8Chen Y, Tang X, Cao X, et al. Human Nogo-C overexpression induces HEK293 cell apoptosis via a mechanism that involves JNK- c-Jun pathway [ J]. Biochem Biophys Res Commun, 2006, 348 (3) : 923-938.
  • 9Oertle T, Merkler D, Schwab ME. Do cancer cells die because of Nogo-B[J]? Oncogene, 2003, 22(2) : 1390-1399.
  • 10Kuang E, Wan Q, Li X, et al. ER stress triggers apoptosis induced by Nogo-B/ASY overexpression[J].Exp Cell Res, 2006, 312(11) : 1983-1988.

引证文献2

二级引证文献1

第四军医大学学报
2006年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部