α-黑素细胞刺激素在自体移植皮片中的mRNA表达及意义

EXPRESSION OF α-MELANOCYTE-STIMULATING HORMONE mRNA AND ITS SIGNIFICANCE IN HUMAN SKIN AUTOGRAFTS

目的初步探索α-黑素细胞刺激素(α-melanocyte-stimulatinghormone,α-MSH)在自体移植皮片过度色素沉着中的作用。方法标本取自颈部自体中厚移植皮片术后1年,局部需再次矫正的18例患者。在切取颈部自体中厚移植皮片同时于每例移植皮片受区及原供区腹部周围分别切取正常皮肤作对照,切取范围1.0cm×0.5cm。分别利用RT-PCR、银染-丽春红染色法计算自体中厚移植皮片、原供区及受区周围自体正常对照皮肤中α-MSHmRNA电泳条带及表皮的灰度值,测定各组中α-MSHmRNA的半定量表达及黑色素的含量。结果α-MSH在自体中厚移植皮片中的mRNA表达明显高于自体正常对照皮肤,差异有统计学意义(P<0.01);自体中厚移植皮片表皮中黑色素含量较自体正常对照皮肤表皮中的黑色素含量明显增多,差异亦有统计学意义(P<0.01);且表皮中黑色素的含量与α-MSH的mRNA表达量呈正相关。结论α-MSH在自体中厚移植皮片中的表达较在自体正常对照皮肤中显著增高,致皮片中黑色素含量升高,在自体移植皮片过度色素沉着中起重要调控作用。

Objective To discuss the expression of α-melanocyte-stimulating hormone （α-MSH） mRNA in human medium thickness skin autografts and to investigate the role of α-MSH in hyperpigmented process of skin autografts. Methods The samples were from medium thickness skin autografts on neck of the patients after 1 year of surgery. The size of sample was about 1.0 cm×0. 5 cm. RT-PCR technique was performed to detect the expression of α-MSH mRNA in medium thickness skin autografts and normal skin respectively. Masson-Fontana stain technique was performed to detect the contents of melanin in medium thickness skin autografts and normal skin epidermis respectively. Results The expression of α-MSH mRNA in medium thickness skin autografts was much stronger than that in control normal skin, showing statistically significant difference （P〈0. 01）. The expression of α-MSH/β2-microglobulin mRNA in normal skin of donor area was no statistically significant differences compared with normal skin around recipient area. The contents of melanin in medium thickness skin autografts epidermis obviously increased when compared with that of control normal skin. The expression of α-MSH mRNA was positive correlated with the contents of melanin in epidermis. Conclusion The above results indicate that the expression of α-MSH increases greater in medium thickness skin autografts than in cortrol samples and was correlated with the pigmentation of skin autografts. Overexpression of α-MSH may play an important role in hyperpigmented process of skin autografts.