毛细管电泳乙腈-盐在线堆积方法机理研究

Studies on Mechanism of Acetonitrile-salts Stacking Method in Capillary Electrophoresis

以两种多肽为例,在详细考察样品基质、背景缓冲液性质以及其它操作因素对堆积影响的基础上,提出乙腈-盐堆积法的可能机理为乙腈-致-快速-大体积堆积-盐诱导-类-等速电泳过程,并用实验加以验证.

Acetonitrile-salt stacking method（ASS）, as a new developed on-line stacking method in capillary electrophoresis, is suitable for stacking and analyzing low concentration analytes, which contain high concen- trations of salts, and it has the huge practical potential in bio-analysis area. However, the applications are limited as the mechanism of ASS is not clear. In this work, the effects of sample matrix, experimental factors ect. on stacking were systematically studied. A new reasonable hypothesis about the mechanism of ASS was proved to be an ＂acetonitrile caused quick large volume stacking combined with NaCl induced pseudo isotachophoresis＂ procedure, and several experiments were carried out to improve the conclusion.