胃癌患者外周血CD4^+CD25^+调节性T细胞的检测及其临床意义

Study on the subset of T lymphocyte and CD4^+CD25^+ regulatory cells in peripheral blood in patients with gastric cancer

目的探讨胃癌患者外周血CD4+CD25+调节性T细胞水平的特点及其临床意义。方法采用流式细胞术检测35例胃癌患者外周血CD4+CD25+调节性T细胞水平并进行分层分析。结果健康对照组CD4+CD25+调节性T细胞水平为(8.69±2.28)%,35例胃癌患者CD4+CD25+调节性T细胞比例为(17.77±8.45)%,统计学有显著差异(P<0.01);进一步分层分析显示随疾病进展外周血CD4+CD25+调节性T细胞水平升高,在III期、IV期尤为明显,统计学有极显著差异(P<0.01)。结论胃癌患者外周血CD4+CD25+调节性T细胞水平的升高与胃癌免疫功能低下及肿瘤的发生发展密切相关,去除这群细胞可有效诱导肿瘤免疫,为肿瘤治疗提供一种新的方法。

Objective：To investigate the changes in peripheral lymphocyte subsets and CD4^＋CD25^＋ regulatory T ceils （ CD4^＋ CD25^＋ Treg） in patients with gastric cancer. Methods： T lymphocyte subset and Treg in 35 patients with gastric cancer were detected by flow cytometry （FCM）. Results： Patients with gastric cancer had a higher proportion of CD4^＋ CD25^＋ Treg cells （ 17.77 ± 8.45 ） % than those of healthy volunteers （ 8.69 ± 2.28 ） % , （ P 〈 0. 05 ） . while in the advanced stages , the expressive rates of CD4^＋ CD25^＋ were significantly increased （P 〈0. O1 ） . Conclnsion：The relative increase in CD4^＋ CD25^＋ regulatory T ceils may be related to and tumor progression in patients with gastric cancer. This finding suggests that the deletion of the CD4^＋ CD25^＋ Treg ceils to treat cancer patients may be an effective strategy .