溃疡性结肠炎患者外周血单个核细胞中Foxp3 mRNA的表达水平

Expression of Foxp3 mRNA in peripheral blood monocytes of patients with ulcerative colitis

目的:探讨溃疡性结肠炎(UC)患者外周血单个核细胞中Foxp3 mRNA表达及其与疾病活动性的关系．方法:应用逆转录-聚合酶链反应(RT-PCR) 检测142例UC患者(活动期22例、缓解期20例) 和30例正常对照组外周血单个核细胞Foxp3 mRNA的表达水平．结果:UC患者急性期Foxp3 mRNA水平低于正常人,差异有显著性(1．58±0．31 vs 3．27± 0．40,P<0．05);缓解期Foxp3 mRNA水平与正常人差异无显著性(P=0．104);PBMC中Foxp3 mRNA表达水平与Walmsley评分标准有相关性,且呈负相关．结论:UC患者外周血单个核细胞中Foxp3 mRNA表达水平显著低于正常人,其参与了 UC的发病过程并与疾病的活动性有关．

AIM： To investigate the level of Foxp3 mRNA in the peripheral blood monocytes （PBMCs） of patients with ulcerative colitis （UC）, and its relationship with activity of UC. METHODS： Reverse transcription-polymerase chain reaction （RT-PCR） was used to examine Foxp3 mRNA expression in the PBMCs from 22 cases with UC in active stage, 20 cases at stable stage, and 30 normal controls. RESULTS： Foxp3 mRNA expression was lower in patients with UC at active stage than that in the controls （1.58 ± 0.31 vs 3.27 ± 0.40, P 〈 0.05）, while Foxp3 mRNA expression in UC at stable stage was not significantly different from that in the controls （P = 0.104）. The level of Foxp3 mRNA in the PBMCs in active UC was negatively correlated with the Walmsley renal scoring （r = -0.756, P = 0.000）. CONCLUSION： Foxp3 mRNA expression in the PBMCs may be involved in the pathogenesis and activity of UC.