胃癌组织Axin蛋白的表达与侵袭转移的相关性

Expression of Axin protein correlates with genesis and metastasis of gastric carcinoma

目的:探讨胃癌组织Axin蛋白的表达与侵袭转移的相关性．方法:胃癌患者46例,均行胃癌根治切除手术．采集胃癌癌组织及远癌正常胃组织标本, 制备石蜡切片,采用免疫组化(SABC)法检测 Axin蛋白的表达,研究其表达和分布特点与临床病理间的关系．结果:正常胃组织Axin蛋白在基底部细胞表达强,表面成熟细胞中表达弱:胃癌组织、远癌正常胃组织中均有Axin蛋白表达,其阳性表达率分别为62．0％和91．3％,差异有统计学意义(P<0．01);Axin蛋白的表达与胃癌临床病理分期(TNM I,ⅡvsⅢ,Ⅳ:78．6％ vs 56．3％, P=0．035)、有无淋巴转移有关(无 vs 有:85．7％ vs 53．1％,P=0．034),与胃癌患者性别、年龄、肿瘤大小、生物学特征和是否侵及浆膜无关(P>0．05)．结论:Axin蛋白表达减弱与胃癌的发生以及肿瘤的临床进展和淋巴转移相关．

AIM： To investigate the relationship between Axin protein expression and genesis and metastasis of gastric carcinoma. METHODS： A total of carcinoma underwent included in this study 46 patients with gastric surgical resection were The expression of Axin protein was detected by immunohistochemical techniques （SABC） in paraffin-embedded samples prepared from gastric carcinoma tissues and normal stomach tissues far from the tumor. In addition, the positive rate of Axin expression was calculated and its correlation with the pathological characteristics of gastric carcinoma was analyzed. RESULTS： In normal stomach tissues, Axin protein expression was strongly positive and intensified in basal cells. Axin was also expressed in tissues from gastric carcinoma and normal stomach mucosa far from the tumor with a positive rate of 62.0% and 91.3%, respectively. There was a significant difference between them （P 〈 0.01）. Axin protein expression was significantly correlated with clinical TNM classification （TNM Ⅰ,Ⅱ vs Ⅲ, Ⅳ： 78.6% vs 56.3%, P = 0.035） and lymphatic metastasis （without vs with： 85.7% vs 53.1%, P = 0.034）. The expression of Axin protein was not markedly correlated with the age and gender of patients, tumor size, biological features and serosa invasion （P 〉 0.05）. CONCLUSION： The expression of Axin protein is down-regulated in gastric carcinoma, which correlates with the genesis and metastasis of the disease.