顶空固相微萃取气相色谱质谱法测定曲药中的香味成分

Study on Flavoring Components of Daqu by Headspace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry(GC-MS)

采用顶空固相微萃取气相色谱-质谱法测定大曲中的香味成分[1]。测定条件为:恒温水浴温度75℃,稳定时间4 h,解吸时间2 min。结果表明,该法可定性大曲中的68种挥发和半挥发性成分;用全扫描采集法,有利于收集到更广泛的非杂环类化合物及其他类物质;成品曲药中的很多微量香味成分如乙醇、己酸、己酸乙酯、乙酸、乙酸乙酯等与基础酒中的微量香味成分相似;大曲中的微量成分的含量远远高于其在基础酒中的含量;初步定性分析了大曲中的部分微量成分,主要是以杂环化合物为主的复合香味成分。(孙悟)

The flavoring components were detected by headspace solid-phase microextraction gas charomatography-mass spectrometry （GC-MS） and the detection conditions were as follows： water bathing temperature at 75℃, 4 h stability time, and 2 min desorption. The results suggested that 68 kinds of volatile components and half-volatile components in Daqu could be determined, the application of full-scan collection method could gather more extensive non-heterocyclic com- pounds and other substances, lots of flavouring components such as ethanol, acetic acid, ethyl caproate, caproic acid, and ethyl acetate etc. in product Daqu were quite similar with the flavouring microconstituents in base liquor, the contents of microconstituents in Daqu were far higher than their contents in base liquor, and the microconstituents in Daqu mainly were heterocyclic compounds through preliminary qualitative analysis.