摘要
木菠萝种子经抽提、40%~60%饱和度硫酸铵沉淀和Gal-Sepharose6B亲和层析,得到纯化的木菠萝凝集素(Jacalin)。Jacalin分子量约为58200,由分子量分别是14000和15800的亚基组成,等电聚焦电泳显示多条区带,其pI为5.7~8.65,Schiff's试剂染色证明它是一种糖蛋白,N-末端为精氨酸和甘氨酸。Jacalin能凝集除山羊、鸡以外的多种动物和人A、B、O和AB血型红细胞,凝集活性受N-酰凝半乳糖胺(GalNAc)和半乳糖(Gal)抑制。在pH值为4.5~10,pH改变不影响Jacalin凝集活性。
The lectin of Jack Fruit (Jacalin) was isolated and purified from the seeds of the Jack Fruit by precipitate of 40% ̄60% saturation ammonium sulfate and affinity chromatography on Gal-Sepharose 6B. Jacalin had an apparent molecular weight of about 58 200 and is composed of two kinds of subunits which had molecular weight 14 000 and 15 800, respectively. Isoelectric focusing on polyacrylamide gel showed that Jacalin gave a spread of components with calculated isoelectric point between 5.7 and 8.65. Jacalin is a glycoprotein by assay of Schiff's stain. The two N-terminals are argenine and glynine. Jacalin could agglutinate red blood cells.
出处
《广西科学》
CAS
1996年第1期35-38,共4页
Guangxi Sciences
基金
国家自然科学基金
关键词
木菠萝凝集素
纯化
性质
凝集素
lectin of Jack Fruit, purification, property