奶水牛血清碱性磷酸酶同工酶的研究

A Study on Serum Alkaline Phosophomonoesterase Isoenzymes in Milk Buffaloes

用聚丙烯酰胺凝胶圆盘电泳和光密度扫描法，对摩拉（Ｍｕｒｒｈ）．尼里（Ｎｉｌｉ－Ｒａｖｉ）及三品系杂交［Ｎｉ♂×（Ｍｕ♂×Ｎａｎｎｉｎｇ♀）♀］母奶水牛血清碱性磷酸酶（ＡＬＰ）同工酶进行研究。结果表明：ＡＬＰ相对总活力为，摩拉（１９６２Ｏ０）＞尼里（１５３４００）＞三品杂（１５０３０Ｏ），尼里为高产奶量组＞低产奶量组、摩拉和三品杂为低产奶量组＞高产奶量组；ＡＬＰ三种同工酶的特点，尼里为ＡＬＰ２＞＞ＡＬＰ１＞ＡＬＰ３、摩拉和三品杂为ＡＬＰ２＞＞ＡＬＰ３＞ＡＬＰ１；种间比较，ＡＬＰ１为尼里＞摩拉＞三品杂、ＡＬＰ２为摩拉＞尼里＞三品杂、ＡＬＰ３则为三品杂＞尼里＞摩拉；种内比较，ＡＬＰ１在尼里和三品杂为低产奶量组＞高产奶量组、在摩拉则为高产奶量组＞低产奶量组，ＡＬＰ２则均为高产奶量组＞低产奶量组，而在ＡＬＰ３则均为低产奶量组＞高产奶量组。实验证实了两性载体电解质为水牛血清ＡＬＰ的强烈抑制剂。

The total relative and relative activity of serum alkaline phosphomonoesterase (ALP)isoenzymes from three breeds of female milk buffaloes, Murrah (Mu), Nili-Ravis (Ni) and a triple crossbred [Tc: Ni♂× (Mu ♂×Nanning♀)♀], was analysed using polyacrylamide disc gel electrophoresis and optical density scanning method. The results showed that the total relative activity of ALP in the three breeds was in the order of Mu (196 200) >Ni (153 400) >Tc (150 300).the isoenzyme activity in the high-milk-yield group was higher than that in the low-yield group in Ni, while the opposite was true in Mu and Tc. ALP2>>ALP1>ALP3 in Ni and ALP2>>ALP3 >ALP1 in Mu and Tc; ALP1: Ni>Mu>Tc, ALP2: Mu>Ni>Tc and ALP3: Tc>Ni>Mu; the activity of ALP1 from low-yield group was higher than that from high-yield group in Ni and Tc,and opposite was true in Mu; the activity of ALP2 from high-yield group was higher than from lowyield group in all the three breeds; the activity of ALP3 was opposite to that of ALP2. The experiment demonstrated that ampholyte carriers were strong inhibitors of serum ALP of buffaloes.