HPLC法测定“心力丸”中华蟾酥毒基和脂蟾毒配基的含量

Determination of cinobufagin and recibufogenin in Xinli pills by HPLC

目的建立测定心力丸中华蟾酥毒基和脂蟾毒配基含量的方法。方法采用反相高效液相色谱法,色谱柱Spherisorb C18(5μm,4.6mm×200 mm),流动相为0.5%(ω)磷酸二氢钾溶液-乙腈(体积比60∶40)(用磷酸调节pH值为3.2),流速1.0 mL/m in,柱温35℃,检测波长为296 nm。结果华蟾酥毒基回收率99.20%,RSD=1.27%;脂蟾毒配基回收率99.40%,RSD=0.86%。结论本法简便、准确,可用于控制该制剂质量。

Objective To establish a method for the content determination of cinobufagin and recibufogenin in Xinli pills. Methods The analysis was performed on a Spherisorb C18 column（ 5μm, 4.6 mm × 200 mm）. The mobile phase consisted of 0. 5% potassium phosphate -acetonitrile （60： 40, pH 3.2）, and the flow rate was 1.0 mL/min. The column temperature was 35 ℃ and detection wavelength 296 nm. Result The average recovery and RSD were 99.20% and 1.27% for cinobufagin, 99.40% and 0. 86% for recibufogenin, respectively. Conclusions This method is simple and reliable for the quality control of Xinli pills.