男性不育精液中NO与UA含量的关系

Relationship between the amount of nitric oxide and uric acid in the semen of infertile men

目的探讨人精液中一氧化氮(NO)与尿酸(UA)含量的关系,及对精子质量的影响。方法参照WHO标准方法,进行精液常规分析,按精子密度、活动率不同分为(正常、＜20、20～40、＞40) 4个组。采用镀酮镉还原荧光法检测NO代谢产物硝酸盐(NO_3^-)。采用尿酶一过氧化物酶偶联法检测精液尿酸含量。结果70例不育组精液中尿酸含量和NO含量为(236．4±47．8)μmol/L、(78．7±1．6)μmol/L与正常生育组(398．6±52．3)μmol/L、(41．8±1．6)μmol/L呈显著性差异(P＜0．01)。将尿酸含量与NO含量进行相关性分析,两者呈显著性负相关(r=-0．96,P＜0．05)。不育各精子密度和活动率组精液尿酸含量随精子密度及活动率增加而上升,NO含量随之下降(P＜0．01)。结论精液尿酸含量测定可作为评价精子受活性氧损害的重要指标,证明尿酸对活性氧尤其在医学领域极为重视的NO损害精子具有保护性作用。

Objective To study the relationship between nitric oxide （NO）, and uric acid （UA） activity of human sperm. Methods Sperm routine analysis was operated refer to the density and activity of sperm. A coppered reduced cadmium fluometric assay was used to detect nitrate, a metabolic product of nitric oxide in sperm. The content of uric acid in semen was measured by uricase-peroxidase method. Results There was significant negative relationship between the content of NO and the density the motility rate, and uric activity of sperms in 70patients （r=- 0.96, P〈0.05）. Conclusion The detection of the activity of sperm uric acid is an effective index to appraise the fertile capability of sperm. NO inhibits the activity of sperm uric acid, and affects the maturity and motility of sperm.