MHC半相合脾加骨髓细胞诱发H22荷瘤鼠的抗肿瘤效应

Graft-versus-tumor activity induced by infusion of MHC half-matched bone marrow cells combined with spleen cells in mice bearing H22 tumor

目的：观察MHC半相合脾加骨髓细胞移植抗小鼠H22实体瘤的效果。方法：以皮下接种H22肝癌细胞的BALB/c×C57BL/6杂交F1代雌性小鼠为受鼠,以健康雌性F1、雄性C57BL/6、雄性C3H小鼠为MHC全相合、半相合、不相合供鼠,观察移植后的抑瘤情况；观察供鼠细胞经^(60)Co照射的MHC半相合移植对WBC、生化和嵌合体的影响；比较供鼠细胞经与不经^(60)Co照射的MHC半相合移植的GVHD情况。结果：供鼠细胞经/不经^(60)Co照射的MHC半相合移植小鼠的肿瘤明显较小,与单纯化疗未进行移植者比较,差异具有统计学意义(P＜0．05)；但受鼠未经化疗预处理的MHC半相合细胞输注没有出现抗肿瘤效应；供鼠细胞经7．5 Gy ^(60)Co照射的MHC半相合移植能明显降低GVHD反应,且对外周血白细胞、生化无不良影响。结论：经7．5 Gy^(60)Co照射的MHC半相合脾加骨髓细胞移植能对H22肝癌细胞产生移植物抗肿瘤效应并降低GVHD反应。

Objective： To observe the anti-tumor effects of transplanting MHC half-matched bone marrow ceils combined with spleen cells in a murine model bearing H22 solid tumor. Methods： Female （BALB/c × C57BL/6） F1 mice （ CB6F1, H-2K^b/d） were subcutaneously inoculated with H22 cells to develop a solid tumor model ; the model mice were taken as recipients. The syngenic, half-matched, and mismatched donor bone marrow ceils were from healthy female F1, male C57BL/6 （H-2Kb）, and male C3H（H-2K） mice, respectively. The tumor weights were measured 18 days after transplantation. The WBC, biochemistry parameters and the formation of chimerism were observed in mice transplanted with MHC half-matched donor cells irradiated by 7.5Gy ^6oCo. Meanwhile, graft-versus-host disease （GVHD） was assessed and the results were compared between mice transplanted with radiated and non-radiated donor cells. Results： The tumor weights of mice transplanted with donor ceils （radiated/non-irradiated by ^6oCo） were significantly decreased com- pared with those of the mice receiving chemotherapy alone（ P 〈 0.05 ）. No anti-tumor effect was found to be induced by MHC half-matched cells in mice receiving no pretreatment of chemotherapy. The transplantation of MHC half-matched donor cells radiated by 7.5 Gy ^6oCo obviously attenuated GVHD and had no adverse effects on peripheral WBC and functions of liver and kidney. Conclusion： Transplantation of 7.5 Gy ^ 6o Co irradiated, MHC half-matched bone marrow ceils combined with spleen cells can induce a graft-versus-tumor effect in mice bearing H22 tumor and can attenuate GVHD.