异基因骨髓胸腺内输注对大鼠小肠移植受者外周血嵌合体影响

The Effect of Injecting Allogene Bone Marrow Into Thymus to Chimera of the Rats Small Intestine Transplantation

目的探讨供体特异性骨髓胸腺内输注大鼠小肠移植后受体大鼠外周血嵌合体动态变化及对急性排斥的影响。方法对18只大鼠进行了供体特异性骨髓细胞(DBMC)输注(试验组),同期18只单纯接受异基因小肠移植(对照组)。采用原位PCR与流式细胞仪相结合的PCR-Flow方法对18只DBMC输注大鼠和18只对照大鼠外周血进行嵌合体检测。结果实验组大鼠术后存活时间(中位数=52.28d,P<0.005)明显延长,病理结果显示排斥反应轻微。可诱导较稳定的移植耐受。移植后5d即有少量嵌合体产生,嵌合状态在15d内呈明显增长,而在15d后嵌合状态增加缓慢。结论胸腺内注射异基因大鼠骨髓细胞可诱导大鼠特异的移植耐受,而外周血嵌合体与耐受状态有明显的相关性。

Objective： To explore dynamic variance of chimera in rats with allogene donor bone marrow cells （DBMC） infusion in recipient＇ s thymus and the impact on rejection. Methods： 18 allograft small intestinal transplantation recipients of preoperative donor bone marrow infusion （experiment group） were prospectively with 18 controls （control g＇roup） given equivalent small intestinal transplantation. Combining situ PCR and flow cytometry （PCR- FLOW） was used, to identify donor derived cell-chirnera in 18 allograft recipients with donor bone marrow infusion （experiment group） and 18 controls （control group） .Results： Survival days of experiment group rats were significantly prolonged （MST = 52.28 days, P 〈 0.005） and have light rejection reaction on pathology. After 15 day of operation, the stable small intestinal transplantation tolerance was established. During the first 5day, the level of chimera was increased with time, afterwards the chimera became relatively stable. Conclusion： Allogenic DMBC injecting in thymus can induce specific transplantation tolerance in rats＇ s small intestinal transplantation. There is a positive correlation of chimera and transplantation tolerance.