绿茶多酚对D-半乳糖与Aβ25～35诱导的AD模型小鼠学习记忆障碍的改善作用

Effects of Green Tea Polyphenol on the Behaviour of Alzheimer's Disease Like Mice Induced by D-galactose and Aβ_(25～35)

目的：建立D-半乳糖与Aβ25～35诱导的AD模型小鼠,观察绿茶多酚对其学习记忆障碍的改善作用.方法：皮下注射D-半乳糖（120 mg/kg,连续给药12 w）,并在第7 w时于侧脑室注射Aβ25～35（4 nmol/L）,建立AD模型小鼠.利用跳台法、水迷宫、避暗法和开场实验评价绿茶多酚对AD模型小鼠学习记忆障碍的改善作用.结果：绿茶多酚明显延长跳台潜伏期,减少跳台错误次数;缩短游出迷宫时间和减少进入盲端的错误次数;减少避暗活动错误次数和延长进入穿梭箱潜伏期;同时,明显增加AD小鼠的探究行为.结论：绿茶多酚对D-半乳糖与Aβ25～35诱导的AD模型小鼠学习记忆障碍具有改善作用.

Objective： To study the effects of green tea polyphenol （GTP） on the behaviour of Alzheimer＇ s Disease like mice induced by D-galactose and Aβ25-35, Methods： D-galactose （120 mg/kg） was intraperitoneally injected into mice for 12 weeks and Aβ25-35 （4nmol） was injected by icv to establish Alzheimer＇ s Disease like mice. The protective and therapeutic effects of GTP were determined by using water maze test, step down test, step through test and open field test, Results： GTP ameliorated the deleterious effects of D-galaetose and Aβ25-35, and thereby improved the animal＇ s learning and memory, prolonged latency time, and the error numbers were significantly reduced. At the same time the autonomic activities were significantly increased. Conclusion： GTP can improve the behaviour of Alzheimer＇ s Disease like mice induced by D-galactose and Aβ25-35.