摘要
目的:研究槐花提取化合物K3的体外抗H IV-1活性,并对其抗H IV-1机制进行初步探讨。方法:采用MTT比色法检测化合物对各种细胞的毒性。用合胞体形成计数法,p24抗原捕获ELISA法及RT-PCR等多种方法研究化合物体外抗H IV-1活性。结论:槐花提取化合物K3体外有较好的抗H IV-1活性,能够抑制病毒实验株(H IV-1ⅢB,耐药株(H IV-174V)和临床分离株(H IV-1KM018)等多种病毒株的复制,且其作用机制是多靶点的,不仅可以抑制病毒的进入,还可以抑制H IV-1逆转录酶活性。
Objective : To investigate the anti-HIV properties of compound K3 purified from Japanese pagoda tree flower as well as its anti-HIV mechanism in vitro. Methods: The cytotoxicity of compound K3 was detected by MTT assay. The anti-HIV activities was measured by syncytium reduction, p24 antigen production and protection for HIV-1 induced cytopathic effects in various HIV-1 strains acute or chronically infected cells system. The mechanism of action of the compound K3 was determined by inhibiting HIV-1 RT activity, cell-to-cell fusion, virus binding and entry into cells. Conclusion: Compound K3 is found to be a potent inhibitor of HIV-1 replication against a various viruses in different cells including laboratory strain (HIV-1 ⅢB ), drug-resistant (HIV-174v ) and clinical isolates (HIV-1KgMO18) of HIV-1. It indicats that K3 act through at least two clarified antiviral mechanisms: inhibition of HIV-1 entry into cell and inhibition of HIV-1 RT activity.
出处
《中药材》
CAS
CSCD
北大核心
2006年第4期355-358,共4页
Journal of Chinese Medicinal Materials
基金
国家高技术研究发展计划(2003AA219142)
国家"十五"科技攻关计划(2004BA719A14)
中国科学院知识创新工程(KSCX2-SW-216KSCX1-SW-11)
云南省自然科学基金(2002C0066M)
云南省科技攻关计划(2004NG12)资助课题