化纤胶囊对肺纤维化大鼠模型肺组织TIMP-1及MMP-2 mRNA表达的影响

Effects of Huaxian Capsule on expression of MMP-2/TIMP-1 Protein and MMP-2 mRNA in lung tissue of pulmonory fibrosis model in rats

目的探讨化纤胶囊对肺纤维化模型大鼠肺组织TIMP-1及MMP-2mRNA表达的影响。方法将Wistar大鼠分为正常组、模型组、中药组(化纤胶囊)、激素组(泼尼松),观察28d。分别于第7、14、28天处死动物,检测组织金属蛋白酶抑制剂(TIMP-1)及基质金属蛋白酶(MMP-2)mRNA蛋白在肺组织的表达,并与模型组进行比较。结果药物组第14、28天TIMP-1、MMP-2mRNA蛋白表达均低与模型组。结论化纤胶囊对TIMP-1、MMP-2mRNA在肺内的异常高表达有抑制作用。

Objective To observe the effects and explore the mechanism of Huaxian Capsule （HXC） on pulmonary fibrosis. Methods 60 rats were divided into the normal group, the HXC group, the prednisone, group, and the model group, 15 rats in each group. The pulmonary fibrosis model of rats was established with bleomycin. The experimental rats were sacrificed in batch on the 7th, 14th and 28th day for determining TIMP-1 protein and MMP-2 mRNA expression by immunohistochemistry and in situ hybridization respectively. The outcomes were compared between groups. Results It was showed that TIMP-1 protein and MMP-2 mRNA expression in different stage of pulmonary fibrosis increased significantly and continuously after modeling, showing significant difference in comparison between the model group and the normal group. After HXC treatment, the two indexes expressed weakly as compared with those in the model group, and showed significant difference to those in the model group on 14th and 28th. Conclusion HXC influence on the expression of TIMP-1 and MMP-2 mRNA in rats with pulmonary fibrosis.