摘要
目的 采用高效液相色谱测定玉红膏中紫草素的含量。方法 选用C18柱,流动相:甲醇-0.05%磷酸水(80:20),检测波长520nm,柱温:30℃。结果 线性范围为0.22~1.1μg(r=0.9998),平均回收率99.4%,RSD=1.8%(n=5)。结论 此方法可以用于玉红膏中紫草素的含量控制。
Objective To determe shikonin in Yuhong ointment by HPLC. Methods C18 Column was used in HPLC with mobile phase:MeOH-H2O(0.05%H3PO4) (80: 20) ,diteetion wavelength:520 nm,eolumn temperature:30℃. Results The linear range of HPLC was 0. 22 - 1. 1 μg ( r = 0. 999 8 ). The average recovery and RSD were 99.4%, 1. 8% respectively ( n = 5 ). Conclusion The method can be used for the quality control of shikonin in Yuhong Oinement.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第4期489-490,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金项目(No.30171145)