气相色谱法测定广藿香中百秋里醇的含量

Determination of Patchouli Alcohol in Herba Pogostemonis by GC

目的 建立广藿香中百秋里醇的气相色谱法（GC）含量测定方法。方法 色谱柱为HP-5柱；柱温采用程序升温，起始150℃，保持23min，8℃／min升至230％，保持2min；气化温度为250％；检测器温度为280℃；分流比为5：1；流速为0．9ml／min。结果 平均回收率为99．31％，RSD为1．89％。14批广藿香的百秋里醇含量在0．259％～0．810％范围之间。结论 方法准确可靠，重复性好，可用于广藿香的质量控制指标之一。

Objective To determine the content of patchouli alcohol in Herba Pogostemonis by GC. Methods HP - 5 column was used. Column temperature was raised by programming. The injector temperature was 250℃ and the detector temperature was 280℃. The column flow was 0.9 ml · min^-1. Results The average recovery of was 99.31% with a RSD of 1.89%. The determination of patchouli alcohol of 14 samples is 0.259% - 0.810%. Conclusion The method is simple and accurate and can be used as a quality control method for Herba Pogostemonis