高效液相色谱法测定甘肃金银花中槲皮素和木樨草素的含量

Determination of Quercetin and Luteolin in Flos Lonicerae Harmsis by HPLC

目的 建立高效液相色谱（HPLC）法测定甘肃金银花中槲皮素和木樨草素含量的方法。方法 采用Kromasil C18柱，以甲醇-水（60：40）为流动相，磷酸调节pH值至3．5，流速：1．0ml·min^-1，检测波长350nm。结果槲皮素在2．93-35．16μg·ml^-1；木樨草素在5．45-43．60μg·ml^-1范围内呈良好线性关系，r槲=0．9999，r木=O．9997。平均回收率：槲皮素为96．2％，RSD=1．7％；木樨草素为96．5％，RSD=2．2％。结论 甘肃金银花中槲皮素和木樨草素的含量远高于正品金银花。该方法灵敏度高，重现性好，可作为金银花质量评价的分析方法。

Objective To establish the method of determine quercetin and luteolin in Flos lonicerae harmsis by HPLC. Methods Kromasil C18Column was used,the mobile phase was consisted of methanol-water （60： 40） ,adjusted pH to 3.5 with H3PO4 ,the flow rate was 1.0 ml·min^-1 and detection wavelength was set at 350 nm. Results The linear range of quercetin was 2.93 - 35.16 μg·ml^-1 , （ r = 0.999 9） and the linear range of luteolin was 5.45 - 43.60 μg·ml^-1 （ r = 0. 999 7 ）, the average recovery and RSD value of Quercetin were 96.2% and 1.7% ; the average recovery and RSD value of luteolin were 96.5% and 2.2%. Conclusion This method is sensitive,accurate and can be used for the quality study of Flos lonicerae.