高效液相色谱法测定萹蓄中金丝桃苷等3种有效成分的含量

Quantitative Determination of Hyperoside and Quercitrin and Luteolin in Polygonum aviculare by HPLC

目的 用高效液相色谱法测定萹蓄中金丝桃苷、槲度苷与木樨草素的含量。方法 采用Shimadzu C18柱（150mm×4．6mm，51μm），流动相为甲醇-0．2％H3PO4溶液（45：55），流速1．0ml·min^-1，检测波长350nm，柱温室温，用外标法定量，同时测定萹蓄中金丝桃苷与槲皮苷的含量；再采用Shimadzu C18柱（150mm×4．6mm，5μm），流动相为甲醇-0．2％H3PO4溶液（50：50），流速1．0ml·min^-1，检测波长254nm，柱温室温，用外标法定量，测定萹蓄中木樨草素的含量。结果 金丝桃苷的线性范围0．50-2．50μg，r=0．9998，回收率为95．13％，RSD为2．9l％；槲皮苷的线性范围0．40-2．00μg，r=0．9996，回收率为96．49％，RSD为2．77％；木樨草素的线性范围0．0325-0．39μg，r=0．9999，回收率为95．75％．RSD为2．49％。结论 该方法简便，快速，线性关系良好，可作为萹蓄的含量测定。

Objective The purpose is to determine the content of hyperoside, quercitrin and luteolin in polygonum aviculare by HPLC. Methods The analysis of hyperoside and quercitrin was carried out on Shimadzu C18column（ 150 mm×4.6 mm,5 μm）. The mobile phase was methanol-0.2% phosphate（45：55 ）. The flow -rate was 1.0 ml·min^-1 and wave -length was 350 nm. Temperature was room temperature. The analysis of luteolin was carried out on Shimadzu C18column（ 150 mm ×4.6 mm,5μm）. The mobile phase was methanol -0.2% phosphate （50： 50）. The flow- rate was 1.0 ml·min^-1 and wave -length was 254 nm. Temperature was room temperature. Results The method was simple and had a good linear relationship. The linear range of hyperoside was 0.50-2.50μg and gave a correlation （r） of 0. 999 8. The recovery was 95.13%. RSD was 2.91%. The linear range of quercitrin was 0.40-2.00μg and gave a correlation （r） of 0.999 6. The recovery was 96.49%. RSD was 2.77%. The linear range of luteolin was 0.40-2.00μg and gave a correlation （r） of 0.999 6. The recovery was 95.75% ,RSD 2.49%. Conclusion The method is easy and fast to determine the content of hypereside, quercitrin and lutcolin from polygonum aviculare.