反相高效液相色谱法测定治偏痛胶囊中芍药苷的含量

Quantitative Determination of Paeoniflorin in Zhipiantong Capsules by High Performance Liquid Chromatography

目的 建立治偏痛胶囊中芍药苷的含量测定方法。方法 采用高效液相色谱法。使用Ultrasphere ODS（250mm×4．6mm，5μm）分析柱，流动相为甲醇-水（35：65），流速为1ml／min，检测波长为230nm，柱温26℃。结果 芍药苷的线性范围为0．47-4．7mg，平均加样回收率为97．3％，RSD为1．23％。制剂中芍药苷的含量限度规定不得少于4mg／粒。结论 方法简便可行，快速准确，重现性好，可很好的应用于治偏痛胶囊中芍药苷的含量测定。

Objetive To establish a reversed-phase HPLC method for the quantitative determination of Paeoniflorin in Zhipiantong Capsules. Methods The experimental conditions were as follows： Uhasphere ODS column （250 mm×4.6 mm,5μm）, mobile phase ： methanol-water（ 35 ： 65 ）, flow rate： 1 ml/min, detective wavelength ： 230 nm, column temperatre： 26℃. Results The calibration curve of Paeoniflorin showed a good linearity within the range of 0.47- 4.7 mg. The regression equation was Y = 1 262 X-39.31 ,being with a correction coefficient of =0. 999 9. The sample was stable in methanol within 24 h. The average recovery was 97.3% and RSD was 1.23%. We propse that the content of Paeoninflorin in the capsules should be no less than 4 mg each. Conclusion The established method is simple, feasible, fast, accurate, reproducible and reliable to operate and suitable for the quality control of Zhipiantong Capsules.