茶多酚对HepG2细胞保护作用的实验研究

Protective Action of Tea Polyphenol on HepG2 Cells

目的:研究茶多酚(TP)对HepG2细胞损伤的保护作用及可能机制。方法:在HepG2细胞培养液中分别加入不同浓度的TP,再加入H2O2及CCl4建立2种损伤模型,通过测定光密度值计算细胞生存率,同时比较天冬氨酸转氨酶(AST)活性的变化。结果:与模型组比较,不同浓度的TP均可提高损伤后的HepG2细胞生存率,降低AST的活性(P<0.01)。结论:TP对HepG2细胞损伤具有保护作用,其机制可能与清除氧自由基、对抗脂质过氧化有关。

OBJECTIVE： To study the protective action of tea phlyphenol（TP） on HepG2 cells injury and the possible mechanism. METHODS： Different concentrations of TP were added into HepG2 cell culture fluid, which was then added with H2O2 and CCl4 to form 2 kinds of injury models, cell survival rate was figured out by detecting the optical density value and changes of AST activities were monitored. RESULTS： As compared with model group, different concentrations of TP increased the survival rate of the injured HepG2 cells, and reduced the activities of AST（P〈0.01）.CONCLUSIONS：TP has protective effect on HepG2 cells injury, the mechanism is possibly related to the clearance of free radicals and its anti- lipid peroxidation.