反相高效液相色谱法测定甘草酸脂质体中甘草酸的含量

Content Determination of Glycyrrhizic Acid in Glycyrrhizin Liposome by RP-HPLC

目的:建立以反相高效液相色谱法测定甘草酸脂质体中甘草酸含量的方法。方法:色谱柱为Shim-PackVP-ODS,流动相为甲醇-磷酸盐缓冲液(pH=2.6,68∶32),检测波长为254nm,流速为1ml/min。结果:甘草酸与辅料及溶剂峰分离良好;甘草酸检测浓度在1～100μg/ml范围内线性关系良好(r=0.9999,n=5);平均回收率为97.56%(RSD=1.03%)。结论:本法准确、灵敏,可用于甘草酸脂质体中甘草酸的含量测定。

OBJECTIVE： To establish an RP HPLC method for the content determination of glycyrrhizic acid in glycyrrhizin liposome. METHODS： The separation was performed on Shim - pack VP - ODS column with methanol - buffer phosphate（pH = 2.6, 68 ： 32） as the mobile phase, the detection wavelength was 254nm and the flow rate was 1ml/min. RESULTS： Glycyrrhizic acid was well -separated with peaks of the adjuvants and the solvent.The linear range for glyeyrrhizic acid was 1-100μg/ml（ r = 0.9 999, n = 5）. The average recovery was 97.56% （RSD = 1.03 % ）. CONCLUSION ： This method is accurate and sensitive,and suitable for the content determination of glycyrrhizic acid in glycyrrhizin liposome.