羟自由基对红细胞膜的损伤作用研究

Study of the hydroxyl free radical oxidative damage of erythrocyte membrane

应用Ｆｅｎｔｏｎ反应系统介导产生的人红细胞体外损伤模型，观察红细胞低渗溶血速率及红细胞ＮＯ２－阴离子通透性的改变。结果显示：低渗溶血通率，ｋ１值在以低剂量Ｈ２Ｏ２（１００ｍｍｏｌ／Ｌ）处理时；与对照组相比有增加，在以高剂量Ｈ２Ｏ２（２００ｍｍｏｌ／Ｌ）处理时则降低，呈双向性反应；ｋ２值各组间经统计学处理均无显著差异。红细胞ＮＯ２－阴离子通透性：反映膜ｂａｕｄ３蛋白的阴离子运输状况，在Ｈ２Ｏ２１００ｍｍｏｌ／Ｌ和２００ｍｍｏｌ／Ｌ两种剂量作用下，通透性均增加。根据以上观察结果，再结合氧化红细胞的形态学改变结果来进行分析，将有助于理解红细胞衰老过程中细胞膜囊泡化脱落，以及衰老抗原的形成。

Oxidative damage of erythrocyte membrane was mediated by Fenton reaction from hydrogen peroxide and FeSO_4/EDTA. The rate of osmotic hemolysis(k) was measured by the method of hypotonic hemolysis.The Band 3 was involved in transport NO_2 across the erythrocyte membrane. k (2～3) and kl values were increased at low(100mmol/L) concentration of H_2O_2 and decreased at high(200mmol/L) concentration (P<0. 01), and the k2 values had no difference as compared with the control. NO_2 permeability across erythrocyte membrane was dramatically increased for both concentrations of H_2O_2. The results may be used to explain the release of spectrin-free vesicles from human erythrocyte and formation of senescent cell antigen during red cell aging.