HPLC法测定苦玄参中苦玄参苷I_A的含量

Measurement of PiefeltarraeninI_A in Picria fel-terrae Lour, by HPLC

目的:建立苦玄参药材Picria fel-terrae Lour．的含量测定方法。方法:RP-HPLC法,采用 C18ODS2柱(5μm,4．6×250mm),乙腈-0．3％磷酸(34:66)为流动相,流速为1mL／min,检测波长为264nm。结果:苦玄参苷IA进样量在0．42μg-2．10μg的范围内呈良好的线性关系,r=0．9999,平均回收率为 100．4％,RSD=1．64％(n=6)。结论:该法操作简便、准确、专属性强,可作为苦玄参药材的质量控制方法。

This article is to establish a method for measuring PiefeltarraeninIA in Picria fel-terrae Lour .Method： RP-HPLC. The PiefeltarraeninIA was separated on a C18ODS2 column（5μm,4.6×250mm） and detected at 264nm and lml/min,using acetonitrile-0.3%phosphoric acid （34：66） as the mobile phase. Result： PiefeharraeninIA in Picria fel-terrae Lour. was bassline separated and determined.The linearity rangs was 0.48μg-2.10μg, correlation coefficient was 0.9999.The average recovery rate was 100.4% （RSD=1.64%, n=6）. Conclusion： The method is simple, accurate and of strong specificity which can be used for quantify the main constituient PiefeharraeninIA in Picria fel-terrae Lour.