摘要
目的:测定炎立消胶囊(丁香叶)中原儿茶酸的含量。方法:HPLC法,色谱柱为Kromasil C18柱(4．6×200mm,5um),流动相为乙腈-水(15:85,V／V),流速为1．0mL．min-1检测波长为260nm．结果:原儿茶酸进样量在0．06-0．18μg范围内呈良好的线性关系,平均回收率为99．8％,RSD为1．2％。结论:本方法简便,重现性好,可作为炎立消胶囊中原儿茶酸的含量测定方法。
Objective To determine the content of protocachuic acid in Yanglixiao capsules （made of clove leaves）.Method HPLC method is used with chromatographic column Krmasil C18（4.6×200mm,5um）,acetonitrile-water（15：85V/V）of mobile phase,1.0mL.min of flow velocity and 260nm of detection wavelength. Result There would be a linear relationship when the application of protocachuic acid ranges within 0.06-0.18ug and in this case the average recovery of this acid can reach 99.8% with 1.2% of RSD .Conclusion This method is simple and convenient to application and therefore it can be used to determine the content of protocachuic acid in Yanlixiao capsules.
出处
《世界科学技术-中医药现代化》
2006年第2期32-34,31,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
