全自动血培养瓶的再利用实验评价

Evaluation on Re-using Experiment of Automatic Blood Culture bottle

目的评价全自动血培养瓶的再利用实验。方法研制高营养快速细菌培养液，并将其应用于全自动血培养仪进行再利用实验，分析阳性率、阳性检出时间和检出菌的种类。结果经59例全自动血培养再利用实验，平均检出时间在再利用血培养瓶与仪器对照组之间无显著的差异（P〉0．05）。608例再利用血培养分离到78株细菌，阳性率12．9％，24h内检出的阳性占64．1％，48h内检出的阳性占87．2％。72h内检出的阳性占96．2％。结论临床应用全自动血培养瓶的再利用实验可提高血培养阳性率。缩短检测时间，减少污染机会。结果快速准确。

Objective To evaluate re-using experiment of automatic blood bottle.Methods Developed high nutrition bacteria medium and re-used it in automatic blood culture system.The positive rate,time to positivity and the type of bacterial were analyzcd.Results After 59 re-using experiments,the positive time of re-using blood culture bottle was not different from those of the control,78 strains were isolated,positive rate were 12.9%,the cumulative positive rates were 64.1% within 24h,87.2% within 48h,96.2% within 72h.Conclusion The positive rate has been raised,the detection time reduced and the chance of contanination decreased by re-using experiment of automatic blood culture bottle.