衍生气相色谱法同时检测分析亚油酸、α-亚麻酸、γ-亚麻酸、AA、EPA、DHA

GC-FID-Derivative Technique Simultaneous Determination of Octadecadienoic Acid,α-Octadecatrienoic Acid,γ-Octadecatrienoic Acid,AA,EPA and DHA

目的 建立衍生气相色谱法同时检测分析亚油酸、α-亚麻酸、γ-亚麻酸、AA、EPA、DHA等六种不饱和脂肪酸。方法 采用KOH-CH3OH甲酯化衍生，利用HP-FFAP 30m×0．32mm×0．25μm毛细管柱分离，FID检测。结果 方法在0-400μg／mL间具有良好的线性（r〉0．999），检出限1μg／mL，回收率91％～103％。结论 本方法可用于食品中含亚油酸、α-亚麻酸、γ-亚麻酸、AA、EPA、DHA等六种不饱和脂肪酸成分的同时酯化衍生分离分析，方法简便，所得结果可靠、重现性好。

Objective To establish a derivative gas chromatography method simultaneously quantitative determination of Octadecadienoic acid, α-Octadecatrienoic Acid, γ- Octadecatrienoic Acid, AA, EPA and DHA altogether six unsaturated fatty acids. Method KOH- CH3OH solution as the esterifying agent, HP-FFAP 30m × 0.32 mm × 0.25 μm capillary column was used to separate the fatty acids and FID for quantitative determination. Result Excellent linearity showed within the range of 0-400 μg/mL （r 〉 0.999）, detection limit was 1 μg/mL, and the recovery rate was 91 -103%. Conclusion The method shows satisfactory repeatability, simplicity and convenience, and is valid in simultaneous derivation separation and analysis of these 6 fatty acids in foodstuff.