咽环部鼻NK/T细胞淋巴瘤中EB病毒和T细胞内抗原1的表达

Epstein Barr Virus Infection and Expression of T-cell Intracellular Antigen-1 (TIA-1) of NK/T-cell Lymphomas in Pharynx Ring

目的探讨咽环部鼻型NK/T细胞淋巴瘤中EB病毒的感染及其T细胞内抗原1(TIA-1)的表达和意义。方法用EBER1/2原位杂交及三步ABC法免疫组织化学染色技术,检测36例咽环部鼻NK/T细胞淋巴瘤患者EB病毒感染及T细胞内抗原1表达情况,选用的抗体有TAI-1、CD56、CD20、CD45RO,并选择10例同时期发生在鼻咽部的鼻咽癌病例做为对照。结果(1)36例病例中,男女之比为3∶1,平均年龄为44岁,以老年人为主。发病部位以鼻咽部最多。(2)36例病例的瘤细胞以大细胞、高度恶性为主,几乎所有病例临床上都有大片凝固性坏死的特点。(3)在36例病例中,EBV检出率为63.89%(23/36),T细胞内抗原1阳性表达率为91.6%(33/36)。10例鼻咽癌病例EBV检出率为80%(8/10)。结论在咽环部鼻NK/T细胞淋巴瘤中存在高水平的EB病毒隐性感染,肿瘤细胞表达TIA-1抗原可能与瘤细胞来源和其特殊的生物学行为有关。

Objective To study status of Epstein Barr virus （EBV） infection and expression of TIA-1 antigen in patients with pharynx ring nasal NK/T-cell lymphomas. Metheds Thirty six patients with pharynx ring nasal NK/T cell lymphornas were received in situ hybridization examination for EBER1/2 through immunohistochemical staining by using the threestep ABC technique for TIA- 1, CD56, CD20, and CD45RO. Selected 10 patients with nasopharyngeal carcinoma were served for comparison. Results （1） Among the 36 patients with pharynx ring nasal NK/T-cell lymphoma, the male to female ratio was 3：1. The average age was 44 years old. Most of them were elderly patients. The lesions were mostly localized within nasopharynx. （2） In 36 cases, most tumor cells were big and malignant. There were frozen putrescence in almost cases. （3） In 23 of the 36 cases （63.89%） EBV signals were detected in the tumor cells; 33 cases expressed TIA - 1 （91.6%, 33/36）. in 8 of 10 cases of nasopharyngeal carcinoma （80%） EBV signals were detected within the tumor cells. Conclusion A high level of latent EBV infection is present in pharynx ring nasal NK/T- cell lymphomas; the expression of TIA - 1 may be related to its cell origin and might responsible for its special biologic behavior too.