直接酶联免疫吸附法检测猪血清IgG的影响因素

Investigation and Optimization of Factors on Measuring IgG in Porcine Serum by Direct Enzyme-Linked Immunoserbent Assay

直接酶联免疫吸附法检测猪血清中G型免疫球蛋白（IgG），最小检测量为10ng，最适检测范围纯IgG为10～60ng，猪血清IgG为10～40ng。酶标抗体最适稀释度为1／3500。酶标板内与酶标板间平行测定变异系数均小于5％。

This paper studied the method of measuring Immunoglobulin G in porcine serum by Direct Enzyme-Linked Immunoserbent Assay. ELISA＇s smallest measurement limit was 10 ng. The best measurement ranges were 10～60 ng for pure IgG and 10～40 ng for porcine serum IgG. The best titer of Peroxidase Conjugate Antibody was 1/3500. Variation coefficient of parallel determination in same and different boards were all less than 5 % .