DPPH法测定葡萄籽原花青素清除自由基的能力

Measuring the Antiradical Efficiency of Proanthocyanidin from Grape Seed by the DPPH·Assay

通过DPPH法测定葡萄籽原花青素抗氧化、清除自由基能力,实验表明:随着葡萄籽原花青素纯化程度的提高,其抗氧化、清除自由基的能力也相应地提高。葡萄籽原花青素纯化物的抗氧化、清除自由基能力大于VC和芦丁,葡萄籽粗提物的抗氧化、清除自由基能力略低于VC,但高于芦丁。GSP、GSPP1、GSPP2、GSPP3、GSPP3-SP、VC、芦丁清除DPPH自由基的半抑制量分别为118,93,88,86,63,110,170 g/kg,其自由基清除能力AE分别为0.84×10-2、1.08×10-2、1.14×10-2、1.16×10-2、1.58×10-2、0.59×10-2、0.59×10-2。

The efficiency of antioxidant and antiradical of the proanthocyanidin extracted from grape seed was determined by the DPPH · assay. It was found that the efficiency increases with the purified degree of the proanthocyanidin from grape seed. The ability of antioxidant and antiradical of the proanthocyanidin from grape seed was greater than those of VC and rutin. The antioxidant and antiradical ability of GSP was slightly lower than those of VC, but higher than those of rutins. EC50 （50% the initial DPPH · concentration） of the GSP, GSPP1, GSPP2, GSPP3,GSPP3-SP, VC and rutin, was 118, 93 , 88, 86, 63, 110, 170 g/kg DPPH · respectively, and the antiradical efficiency （AE） was 0.84 × 10^-2 ,1.08 × 10^-2 ,1.14 × 10^-2 ,1.16 × 10^-2 ,1.58 × 10^-2 ,0.59 × 10^-2 ,0.59 × 10^-2.