CglI基因在大肠杆菌中的功能活性分析

Functional Activity Analysis of CglI Gene in E.coli Strain

通过PCR技术从谷氨酸棒杆菌基因组中扩增CglI基因,克隆到载体pMD18-T Simple后测序。将CglI基因亚克隆到表达载体pJL23,构建重组质粒pJL23-CglI,转化大肠杆菌HB101菌株,通过PCR反应筛选鉴定阳性克隆。通过噬菌体感染实验,初步分析了CglI基因在大肠杆菌中的功能活性。

CglI gene from Conynebacterium glutamicum genome was amplified with PCR and cloned into vector pMDIS-T Simple to identify its fragment size and gene sequence. The recombinant plasmid pJL23-CglI was constructed by subcloning of Cgl I gene to express vector pJ L23 and then transformed to E. coli HB101 strain. The positive cloned strains were screened and identified by PCR. The functional activity of CglI gene in E. coli was analyzed with bacteriophage infection.