摘要
从六安产甘蔗病叶中提取总RNA,逆转录获得甘蔗花叶病毒(Sorghun mosaic virus,SrMV)基因组cDNA。设计P3基因特异引物,通过PCR扩增P3基因,并将P3基因cDNA克隆、测序。
The total RNA was extracted from sugarcane leave originated in Lu'an City. Sorghun mosaic virus (SrMV)genome cDNA was obtained by reverse transcription. Special primers for SrMV P3 gene were designed and P3 gene was cloned and sequeced.
出处
《皖西学院学报》
2006年第2期62-65,共4页
Journal of West Anhui University
基金
皖西学院2005年度应用科研项目(WXZY0503)提供资助