应用酶联免疫吸附试验检测血清中抗巨细胞病毒IgM

Determination of The Anti-cytomegalovirus IgM by Using Enzyme-linked Immunosorbent Assay (ELISA)

本文应用酶联免疫吸附试验(ELISA)法检测了单项丙氨酸氨基转移酶(ALT)升高患者血清115份,病毒性肝炎患者血清56份,正常人血清170份,血清中抗-CMV-IgM阳性率分别为37.4%、19.6%和8.2%.对单项ALT升高而临床诊断不明的病人有必要进行抗-CMV-IgM检测;对于病毒性肝炎患者,CMV 感染在特定情况下可加重病情,故检测病人血清抗-CMV-IgM有一定临床意义;由于献血员中存在着CMV较高带毒率,在免疫缺陷病人、器官移植者及婴儿等特殊易感人群输血时,应加强对献血者CMV检测和筛选.

Serums from 341 people were tested by using ELISA. Among them ,115 were from people whose Alanine Aminotransferase (ALT) were singly high, 56 were from patients with viral hepatitis,and 170 were from healthy controls. The positive rates of anti-cytomegalovirus IgM (An-ti-CMV IgM) in these serums were 37. 4% ,19.6% and 8. 2% respectively. Anti-CMV IgM testing was necessary for those patients whose ALT was singly high but the clinical diagnosis was uncertain. This testing was also significant for patients with viral hepatitis since the infection of the cy-tomegalovirus (CMV) may worsen the condition of the patients under a certain circumstance. As the rate of virus-carrying blood donators is high,blood transfusion is dangerous to those people who are particularly susceptible to the virus,such as immunodeficiencies,organ transplantation, and infants, the determination of the CMV and the selection of the blood donators should be strengthened.